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top2 isoforms  (Inspiralis Ltd)

 
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    Inspiralis Ltd top2 isoforms
    <t>TOP2</t> assays. TOP2 activity was analyzed by decatenation assay using purified TOP2 <t>isoforms</t> and catenated DNA. Upper panels show quantification of visualized gels of the inhibition by dexrazoxane (A) and XK469 (B). Lower panels show representative gels of the inhibition of TOP2A (C) and TOP2B (D). TARDIS assay of TOP2-DNA covalent complexes in neonatal cardiomyocytes (E) and wild-type HL-60 cells (F) were incubated with increasing concentrations of either dexrazoxane or XK469 for 2 h. About 100 µM ETO was used as the positive control. Data are expressed as median with an interquartile range of intensities of the individual images. Statistical analyses: n = 4, 1-way ANOVA, Holm-Sidak’s post hoc test, p ≤ .05, “e”—compared with a positive control (100 µM ETO).
    Top2 Isoforms, supplied by Inspiralis Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/top2 isoforms/product/Inspiralis Ltd
    Average 90 stars, based on 1 article reviews
    top2 isoforms - by Bioz Stars, 2026-04
    90/100 stars

    Images

    1) Product Images from "Exploring the effects of topoisomerase II inhibitor XK469 on anthracycline cardiotoxicity and DNA damage"

    Article Title: Exploring the effects of topoisomerase II inhibitor XK469 on anthracycline cardiotoxicity and DNA damage

    Journal: Toxicological Sciences

    doi: 10.1093/toxsci/kfae008

    TOP2 assays. TOP2 activity was analyzed by decatenation assay using purified TOP2 isoforms and catenated DNA. Upper panels show quantification of visualized gels of the inhibition by dexrazoxane (A) and XK469 (B). Lower panels show representative gels of the inhibition of TOP2A (C) and TOP2B (D). TARDIS assay of TOP2-DNA covalent complexes in neonatal cardiomyocytes (E) and wild-type HL-60 cells (F) were incubated with increasing concentrations of either dexrazoxane or XK469 for 2 h. About 100 µM ETO was used as the positive control. Data are expressed as median with an interquartile range of intensities of the individual images. Statistical analyses: n = 4, 1-way ANOVA, Holm-Sidak’s post hoc test, p ≤ .05, “e”—compared with a positive control (100 µM ETO).
    Figure Legend Snippet: TOP2 assays. TOP2 activity was analyzed by decatenation assay using purified TOP2 isoforms and catenated DNA. Upper panels show quantification of visualized gels of the inhibition by dexrazoxane (A) and XK469 (B). Lower panels show representative gels of the inhibition of TOP2A (C) and TOP2B (D). TARDIS assay of TOP2-DNA covalent complexes in neonatal cardiomyocytes (E) and wild-type HL-60 cells (F) were incubated with increasing concentrations of either dexrazoxane or XK469 for 2 h. About 100 µM ETO was used as the positive control. Data are expressed as median with an interquartile range of intensities of the individual images. Statistical analyses: n = 4, 1-way ANOVA, Holm-Sidak’s post hoc test, p ≤ .05, “e”—compared with a positive control (100 µM ETO).

    Techniques Used: Activity Assay, Purification, Inhibition, Incubation, Positive Control

    TOP2B proteasomal degradation in rat neonatal cardiomyocytes . Cells were treated with increasing concentrations of dexrazoxane (DEX) (A) or XK469 (B) for 24 h. Then, TOP2 content in cells was evaluated by immunodetection. Statistical analyses: n = 3–4, mean ± SD, 1-way ANOVA, Holm-Sidak’s post hoc test, p ≤ .05, “c”—compared with drug-free control.
    Figure Legend Snippet: TOP2B proteasomal degradation in rat neonatal cardiomyocytes . Cells were treated with increasing concentrations of dexrazoxane (DEX) (A) or XK469 (B) for 24 h. Then, TOP2 content in cells was evaluated by immunodetection. Statistical analyses: n = 3–4, mean ± SD, 1-way ANOVA, Holm-Sidak’s post hoc test, p ≤ .05, “c”—compared with drug-free control.

    Techniques Used: Immunodetection, Control



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    top2 isoforms  (Inspiralis Ltd)
    90
    Inspiralis Ltd top2 isoforms
    <t>TOP2</t> assays. TOP2 activity was analyzed by decatenation assay using purified TOP2 <t>isoforms</t> and catenated DNA. Upper panels show quantification of visualized gels of the inhibition by dexrazoxane (A) and XK469 (B). Lower panels show representative gels of the inhibition of TOP2A (C) and TOP2B (D). TARDIS assay of TOP2-DNA covalent complexes in neonatal cardiomyocytes (E) and wild-type HL-60 cells (F) were incubated with increasing concentrations of either dexrazoxane or XK469 for 2 h. About 100 µM ETO was used as the positive control. Data are expressed as median with an interquartile range of intensities of the individual images. Statistical analyses: n = 4, 1-way ANOVA, Holm-Sidak’s post hoc test, p ≤ .05, “e”—compared with a positive control (100 µM ETO).
    Top2 Isoforms, supplied by Inspiralis Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/top2 isoforms/product/Inspiralis Ltd
    Average 90 stars, based on 1 article reviews
    top2 isoforms - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    TOP2 assays. TOP2 activity was analyzed by decatenation assay using purified TOP2 isoforms and catenated DNA. Upper panels show quantification of visualized gels of the inhibition by dexrazoxane (A) and XK469 (B). Lower panels show representative gels of the inhibition of TOP2A (C) and TOP2B (D). TARDIS assay of TOP2-DNA covalent complexes in neonatal cardiomyocytes (E) and wild-type HL-60 cells (F) were incubated with increasing concentrations of either dexrazoxane or XK469 for 2 h. About 100 µM ETO was used as the positive control. Data are expressed as median with an interquartile range of intensities of the individual images. Statistical analyses: n = 4, 1-way ANOVA, Holm-Sidak’s post hoc test, p ≤ .05, “e”—compared with a positive control (100 µM ETO).

    Journal: Toxicological Sciences

    Article Title: Exploring the effects of topoisomerase II inhibitor XK469 on anthracycline cardiotoxicity and DNA damage

    doi: 10.1093/toxsci/kfae008

    Figure Lengend Snippet: TOP2 assays. TOP2 activity was analyzed by decatenation assay using purified TOP2 isoforms and catenated DNA. Upper panels show quantification of visualized gels of the inhibition by dexrazoxane (A) and XK469 (B). Lower panels show representative gels of the inhibition of TOP2A (C) and TOP2B (D). TARDIS assay of TOP2-DNA covalent complexes in neonatal cardiomyocytes (E) and wild-type HL-60 cells (F) were incubated with increasing concentrations of either dexrazoxane or XK469 for 2 h. About 100 µM ETO was used as the positive control. Data are expressed as median with an interquartile range of intensities of the individual images. Statistical analyses: n = 4, 1-way ANOVA, Holm-Sidak’s post hoc test, p ≤ .05, “e”—compared with a positive control (100 µM ETO).

    Article Snippet: We assessed TOP2 activity using both TOP2 isoforms (purchased from Inspiralis, Inc.) using catenated DNA as a substrate.

    Techniques: Activity Assay, Purification, Inhibition, Incubation, Positive Control

    TOP2B proteasomal degradation in rat neonatal cardiomyocytes . Cells were treated with increasing concentrations of dexrazoxane (DEX) (A) or XK469 (B) for 24 h. Then, TOP2 content in cells was evaluated by immunodetection. Statistical analyses: n = 3–4, mean ± SD, 1-way ANOVA, Holm-Sidak’s post hoc test, p ≤ .05, “c”—compared with drug-free control.

    Journal: Toxicological Sciences

    Article Title: Exploring the effects of topoisomerase II inhibitor XK469 on anthracycline cardiotoxicity and DNA damage

    doi: 10.1093/toxsci/kfae008

    Figure Lengend Snippet: TOP2B proteasomal degradation in rat neonatal cardiomyocytes . Cells were treated with increasing concentrations of dexrazoxane (DEX) (A) or XK469 (B) for 24 h. Then, TOP2 content in cells was evaluated by immunodetection. Statistical analyses: n = 3–4, mean ± SD, 1-way ANOVA, Holm-Sidak’s post hoc test, p ≤ .05, “c”—compared with drug-free control.

    Article Snippet: We assessed TOP2 activity using both TOP2 isoforms (purchased from Inspiralis, Inc.) using catenated DNA as a substrate.

    Techniques: Immunodetection, Control